Aims: Human Papilloma Virus (HPV) is known as one of the causes of anogenital cancers, including cervix cancer. The inability of viral culture has made the initial detection of HPV difficult. Therefore, molecular methods are the best way to identify, and among these methods, Polymerase Chain Reaction (PCR) amplification methods are very useful and accurate in diagnosis. The aim of this study was to investigate the presence of HPV in Iranian patients and the relationship between the type of virus (high risk and low risk) and cervical lesions by molecular methods based on PCR virus samples.
Materials and Methods: The present study was conducted on 67 biopsy and Liquid base cytological samples including cervical Liquid cytology and cervical tissue biopsy. The cytological diagnostic test and pathological examination were performed and the DNA was extracted and then, DNA typing was conducted by PCR using primers MY09/MY11, specific for capsid gene.
Findings: Out of 67 patients, 2 cases (3.0%) were not infected with HPV, while cervical cytology was symptomatic. Seven cases had no symptoms in the cervical epithelial cells, which were virtually positive for the virus, and the rest also had both viral and clinical symptoms. Most of the samples (52.2%) were infected with high-risk viruses and, most of them were HPV16.
Conclusion: HPV can be the main responsible for the cervical cells changes, that has the ability to become cancerous. Also, in most cases, the main cause of the infection is high-risk type of the virus. The most prevalent type of HPV that infected cervical epithelial cells is HPV16.