دوره 7، شماره 3 - ( 1401 )                   دوره 7 شماره 3 صفحات 164-157 | برگشت به فهرست نسخه ها


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Sanaye Naderi M, Nateghi M R. Investigating the expression of mesenchymal markers in stem cells of human umbilical cord matrix (Wharton's jelly). SJMR 2022; 7 (3) : 4
URL: http://saremjrm.com/article-1-274-fa.html
امینی مهابادی جواد، صنایع نادری مریم، ناطقی محمد رضا. بررسی بیان مارکرهای مزانشیمال در سلول های بنیادی ماتریکس بندناف انسان (ژله وارتون). مجله تحقيقات پزشكي صارم. 1401; 7 (3) :157-164

URL: http://saremjrm.com/article-1-274-fa.html


1- مرکز تحقیقات آناتومی، دانشگاه علوم پزشکی کاشان، کاشان-ایران
2- مرکز تحقیقات زنان، زایمان و ناباروری صارم، بیمارستان فوق تخصصی صارم، دانشگاه علوم پزشکی ایران، تهران، ایران
چکیده:   (1164 مشاهده)
مقدمه: سلول­های بنیادی مزانشیمی سلول­های چندتوانی هستند که از ویژ­گی آن ها داشتن توانایی خودبازسازی و قابلیت تمایز و تبدیل شدن به انواع دیگر سلول­های بدن می باشد. سلول­های بند ناف بعنوان یک منبع با ارزشی از سلول­های بنیادی مزانشیمی بوده که برای درمان سلول­های بنیادی مورد توجه هستند. در این مطالعه با استفاده از چند مارکر مزانشیمال، سلول­های بنیادی ماتریکس بند ناف بررسی گردید.
مواد و روش ها: در تحقیق حاضر پس از جداسازی و کشت سلول­ها وقتی به تراکم 80 درصد رسیدند ترپسینه شده و پاساژ داده شدند و بعد از پاساژ چهارم سلول­ها آماده فلوسایتومتری شدند. ابتدا سلول­ها شمارش شدند و حدود 106×5 سلول همراه با 20 میکرولیتر آنتی بادی کونژوگه شده بافیکواریترین (PE) و فلورسین ایزوتیوسیانات (FITC) و 100 ماکرولیتر  PBSآماده سازی شد و سانتریفیوژ گردید. در نهایت، پلاک سلولی در 100 ماکرولیتر  PBSحل و با دستگاه فلوسایتومتری آنالیز شد.
نتایج: یافته­ها نشان داد بیش از 80 درصد جمعیت سلول بنیادی مشتق شده از ماتریکس بند ناف، مارکرهای سلول­های بنیادی مزانشیمی را بیان کردند. جمعیت سلول­های بنیادی جدا شده از ماتریکس بند ناف برای مارکرهای سلول­های بنیادی مزانشیمی مثبت بودند.
نتیجه گیری: نتایج نشان می­دهد که سلول­های ماتریکس بند ناف منبعی از سلول­های بنیادی مزانشیمال بوده که می­تواند جایگزین مناسبی برای سلول­های بنیادی در کاربردهای کلینیکی و سلول درمانی باشد
 
شماره‌ی مقاله: 4
متن کامل [PDF 387 kb]   (280 دریافت)    
نوع مقاله: پژوهشی اصيل | موضوع مقاله: تولیدمثل
دریافت: 1401/8/15 | پذیرش: 1401/9/15 | انتشار: 1402/3/24

فهرست منابع
1. Mahabadi, J.A., et al., Derivation of male germ cells from induced pluripotent stem cells by inducers: A review. Cytotherapy, 2018. 20(3): 279-290. [DOI:10.1016/j.jcyt.2018.01.002]
2. Baksh, D., L. Song, and R.S. Tuan, Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. Journal of cellular and molecular medicine, 2004. 8(3): 301-316. [DOI:10.1111/j.1582-4934.2004.tb00320.x]
3. Barry, F.P. and J.M. Murphy, Mesenchymal stem cells: clinical applications and biological characterization. The international journal of biochemistry & cell biology, 2004. 36(4): 568-584. [DOI:10.1016/j.biocel.2003.11.001]
4. Tropel, P., et al., Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Experimental cell research, 2004. 295(2): 395-406. [DOI:10.1016/j.yexcr.2003.12.030]
5. ML, T.D.W., Wharton's jelly-derived cells are a primitive stromal cell population Stem Cells 26591 2008. 9. Troyer, DL, and Weiss, ML Wharton's jelly-derived cells are a primitive stromal cell population. Stem Cells, 2008. 26: 591. [DOI:10.1634/stemcells.2007-0439]
6. Fu, Y.-S., et al., Conversion of human umbilical cord mesenchymal stem cells in Wharton's jelly to dopaminergic neurons in vitro: potential therapeutic application for Parkinsonism. Stem cells, 2006. 24(1): 115-124. [DOI:10.1634/stemcells.2005-0053]
7. Baksh, D., R. Yao, and R.S. Tuan, Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow. Stem cells, 2007. 25(6): 1384-1392. [DOI:10.1634/stemcells.2006-0709]
8. Wang, J.-F., et al., Mesenchymal stem/progenitor cells in human umbilical cord blood as support for ex vivo expansion of CD34 (+) hematopoietic stem cells and for chondrogenic differentiation. haematologica, 2004. 89(7): 837-844.
9. Sarugaser, R., et al., Human umbilical cord perivascular (HUCPV) cells: a source of mesenchymal progenitors. Stem cells, 2005. 23(2): 220-229. [DOI:10.1634/stemcells.2004-0166]
10. Weiss, M.L., et al., Human umbilical cord matrix stem cells: preliminary characterization and effect of transplantation in a rodent model of Parkinson's disease. Stem cells, 2006. 24(3): 781-792. [DOI:10.1634/stemcells.2005-0330]
11. Qiao, C., et al., Human mesenchymal stem cells isolated from the umbilical cord. Cell biology international, 2008. 32(1): 8-15. [DOI:10.1016/j.cellbi.2007.08.002]
12. Erices, A., P. Conget, and J.J. Minguell, Mesenchymal progenitor cells in human umbilical cord blood. British journal of haematology, 2000. 109(1): 235-242. [DOI:10.1046/j.1365-2141.2000.01986.x]
13. Jiang, Y., et al., Pluripotency of mesenchymal stem cells derived from adult marrow. Nature, 2002. 418(6893): 41-49. [DOI:10.1038/nature00870]
14. Troyer DLꎬWeiss, M., Wharton′ s jelly-derived cells are a primitive stromal cell population. Stem Cellsꎬ2008ꎬ26 (3): 591-599. [DOI:10.1634/stemcells.2007-0439]
15. Lu, L.-L., et al., Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials. haematologica, 2006. 91(8): 1017-1026.
16. Secco, M., et al., Multipotent stem cells from umbilical cord: cord is richer than blood! Stem cells, 2008. 26(1): 146-150. [DOI:10.1634/stemcells.2007-0381]
17. Can, A. and S. Karahuseyinoglu, Concise review: human umbilical cord stroma with regard to the source of fetus-derived stem cells. Stem cells, 2007. 25(11): 2886-2895. [DOI:10.1634/stemcells.2007-0417]
18. Karahuseyinoglu, S., et al., Biology of stem cells in human umbilical cord stroma: in situ and in vitro surveys. Stem cells, 2007. 25(2): 319-331. [DOI:10.1634/stemcells.2006-0286]
19. Kern, S., et al., Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem cells, 2006. 24(5): 1294-1301. [DOI:10.1634/stemcells.2005-0342]
20. Jones, E. and D. McGonagle, Human bone marrow mesenchymal stem cells in vivo. Rheumatology, 2008. 47(2): 126-131. [DOI:10.1093/rheumatology/kem206]
21. Solter, D. and B.B. Knowles, Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1). Proceedings of the National Academy of Sciences, 1978. 75(11): 5565-5569. [DOI:10.1073/pnas.75.11.5565]
22. Wiesmann, A., et al., Decreased CD90 expression in human mesenchymal stem cells by applying mechanical stimulation. Head & face medicine, 2006. 2: 1-6. [DOI:10.1186/1746-160X-2-8]
23. Jin, H.J., et al., Down-regulation of CD105 is associated with multi-lineage differentiation in human umbilical cord blood-derived mesenchymal stem cells. Biochemical and biophysical research communications, 2009. 381(4): 676-681. [DOI:10.1016/j.bbrc.2009.02.118]
24. Koch, T.G., et al., Isolation of mesenchymal stem cells from equine umbilical cord blood. BMC biotechnology, 2007. 7: 1-9. [DOI:10.1186/1472-6750-7-26]
25. Lovati, A.B., et al., Comparison of equine bone marrow-, umbilical cord matrix and amniotic fluid-derived progenitor cells. Veterinary research communications, 2011. 35: 103-121. [DOI:10.1007/s11259-010-9457-3]
26. Seo, M.J., et al., Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo. Biochemical and biophysical research communications, 2005. 328(1): 258-264. [DOI:10.1016/j.bbrc.2004.12.158]
27. Romanov, Y.A., V.A. Svintsitskaya, and V.N. Smirnov, Searching for alternative sources of postnatal human mesenchymal stem cells: candidate MSC-like cells from umbilical cord. Stem cells, 2003. 21(1): 105-110. [DOI:10.1634/stemcells.21-1-105]
28. Zeddou, M., et al., The umbilical cord matrix is a better source of mesenchymal stem cells (MSC) than the umbilical cord blood. Cell biology international, 2010. 34(7): 693-701. [DOI:10.1042/CBI20090414]
29. Mahabadi, J.A., et al., Derivation of male germ cells from induced pluripotent stem cells by inducers: A review. Cytotherapy, 2018. 20(3): 279-290. [DOI:10.1016/j.jcyt.2018.01.002]
30. Baksh, D., L. Song, and R.S. Tuan, Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. Journal of cellular and molecular medicine, 2004. 8(3): 301-316. [DOI:10.1111/j.1582-4934.2004.tb00320.x]
31. Barry, F.P. and J.M. Murphy, Mesenchymal stem cells: clinical applications and biological characterization. The international journal of biochemistry & cell biology, 2004. 36(4): 568-584. [DOI:10.1016/j.biocel.2003.11.001]
32. Tropel, P., et al., Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Experimental cell research, 2004. 295(2): 395-406. [DOI:10.1016/j.yexcr.2003.12.030]
33. ML, T.D.W., Wharton's jelly-derived cells are a primitive stromal cell population Stem Cells 26591 2008. 9. Troyer, DL, and Weiss, ML Wharton's jelly-derived cells are a primitive stromal cell population. Stem Cells, 2008. 26: 591. [DOI:10.1634/stemcells.2007-0439]
34. Fu, Y.-S., et al., Conversion of human umbilical cord mesenchymal stem cells in Wharton's jelly to dopaminergic neurons in vitro: potential therapeutic application for Parkinsonism. Stem cells, 2006. 24(1): 115-124. [DOI:10.1634/stemcells.2005-0053]
35. Baksh, D., R. Yao, and R.S. Tuan, Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow. Stem cells, 2007. 25(6): 1384-1392. [DOI:10.1634/stemcells.2006-0709]
36. Wang, J.-F., et al., Mesenchymal stem/progenitor cells in human umbilical cord blood as support for ex vivo expansion of CD34 (+) hematopoietic stem cells and for chondrogenic differentiation. haematologica, 2004. 89(7): 837-844.
37. Sarugaser, R., et al., Human umbilical cord perivascular (HUCPV) cells: a source of mesenchymal progenitors. Stem cells, 2005. 23(2): 220-229. [DOI:10.1634/stemcells.2004-0166]
38. Weiss, M.L., et al., Human umbilical cord matrix stem cells: preliminary characterization and effect of transplantation in a rodent model of Parkinson's disease. Stem cells, 2006. 24(3): 781-792. [DOI:10.1634/stemcells.2005-0330]
39. Qiao, C., et al., Human mesenchymal stem cells isolated from the umbilical cord. Cell biology international, 2008. 32(1): 8-15. [DOI:10.1016/j.cellbi.2007.08.002]
40. Erices, A., P. Conget, and J.J. Minguell, Mesenchymal progenitor cells in human umbilical cord blood. British journal of haematology, 2000. 109(1): 235-242. [DOI:10.1046/j.1365-2141.2000.01986.x]
41. Jiang, Y., et al., Pluripotency of mesenchymal stem cells derived from adult marrow. Nature, 2002. 418(6893): 41-49. [DOI:10.1038/nature00870]
42. Troyer DLꎬWeiss, M., Wharton′ s jelly-derived cells are a primitive stromal cell population. Stem Cellsꎬ2008ꎬ26 (3): 591-599. [DOI:10.1634/stemcells.2007-0439]
43. Lu, L.-L., et al., Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials. haematologica, 2006. 91(8): 1017-1026.
44. Secco, M., et al., Multipotent stem cells from umbilical cord: cord is richer than blood! Stem cells, 2008. 26(1): 146-150. [DOI:10.1634/stemcells.2007-0381]
45. Can, A. and S. Karahuseyinoglu, Concise review: human umbilical cord stroma with regard to the source of fetus-derived stem cells. Stem cells, 2007. 25(11): 2886-2895. [DOI:10.1634/stemcells.2007-0417]
46. Karahuseyinoglu, S., et al., Biology of stem cells in human umbilical cord stroma: in situ and in vitro surveys. Stem cells, 2007. 25(2): 319-331. [DOI:10.1634/stemcells.2006-0286]
47. Kern, S., et al., Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem cells, 2006. 24(5): 1294-1301. [DOI:10.1634/stemcells.2005-0342]
48. Jones, E. and D. McGonagle, Human bone marrow mesenchymal stem cells in vivo. Rheumatology, 2008. 47(2): 126-131. [DOI:10.1093/rheumatology/kem206]
49. Solter, D. and B.B. Knowles, Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1). Proceedings of the National Academy of Sciences, 1978. 75(11): 5565-5569. [DOI:10.1073/pnas.75.11.5565]
50. Wiesmann, A., et al., Decreased CD90 expression in human mesenchymal stem cells by applying mechanical stimulation. Head & face medicine, 2006. 2: 1-6. [DOI:10.1186/1746-160X-2-8]
51. Jin, H.J., et al., Down-regulation of CD105 is associated with multi-lineage differentiation in human umbilical cord blood-derived mesenchymal stem cells. Biochemical and biophysical research communications, 2009. 381(4): 676-681. [DOI:10.1016/j.bbrc.2009.02.118]
52. Koch, T.G., et al., Isolation of mesenchymal stem cells from equine umbilical cord blood. BMC biotechnology, 2007. 7: 1-9. [DOI:10.1186/1472-6750-7-26]
53. Lovati, A.B., et al., Comparison of equine bone marrow-, umbilical cord matrix and amniotic fluid-derived progenitor cells. Veterinary research communications, 2011. 35: 103-121. [DOI:10.1007/s11259-010-9457-3]
54. Seo, M.J., et al., Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo. Biochemical and biophysical research communications, 2005. 328(1): 258-264. [DOI:10.1016/j.bbrc.2004.12.158]
55. Romanov, Y.A., V.A. Svintsitskaya, and V.N. Smirnov, Searching for alternative sources of postnatal human mesenchymal stem cells: candidate MSC-like cells from umbilical cord. Stem cells, 2003. 21(1): 105-110. [DOI:10.1634/stemcells.21-1-105]
56. Zeddou, M., et al., The umbilical cord matrix is a better source of mesenchymal stem cells (MSC) than the umbilical cord blood. Cell biology international, 2010. 34(7): 693-701. [DOI:10.1042/CBI20090414]

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