Volume 1, Issue 3 (2017)                   SJRM 2017, 1(3): 89-95 | Back to browse issues page


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Mojtahedi F, Pooladi A, Sirati F, Kaihani E, Akhlaghpour S, Karimlou M, et al . Abberant Lymphocytes Rate after Gamma-Irradiationn as a Biomarker of Breast Cancer. SJRM. 2017; 1 (3) :89-95
URL: http://saremjrm.com/article-1-35-en.html
1- Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran
2- “Sarem Fertility & Infertility Research Center (SAFIR)” and “Sarem Cell Research Center (SCRC)”, Tehran, Iran
3- Surgery Department, Medicine Faculty, Tehran University of Medical Sciences, Tehran, Iran
4- Navid Medical Center, Tehran, Iran
5- Statistics Department, Medicine Faculty, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran
6- “Sarem Cell Research Center (SCRC)” and “Medical Genetics Department”, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran
7- Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran , f_behjati@uswr.ac.ir
Abstract:   (4036 Views)

Aims: The use of non-invasive laboratory tests based on detection of biomarkers in the blood samples is a good strategy for early diagnosis of breast cancer. The lymphocyte radiosensitivity assessment can be a valuable method to diagnose breast cancer. The objective of this study was to investigate the radiosensitivity indices in sporadic breast cancer among Iranian women and to evaluate the potential of those indices for clinical use in early diagnosis of breast cancer and assessing its susceptibility.
Materials and Methods: The present study is a case-control that was conducted on 32 patients with sporadic breast cancer (patient group) and 30 healthy individuals (control group). The obtained blood samples of both groups were exposed to gamma-irradiation (0.4 Gy) and the level of chromosome breakage was determined based on the G2 chromosome breakage assay protocol. In the metaphase lymphocytes, the percentage of the abnormal cells was calculated as the radiosensitivity index for comparing the two groups. Data were analyzed by SPSS 19 software using student t-test, paired t-test, chi-square and Fisher exact test.
Findings: There was a significant difference in the percentage of the index of the abnormal cells after irradiation between two groups (p=0.001). The area under the curve (AUC) of the percentage of the abnormal cells and odds ratio (OR) were found as 0.725 with 3.818, respectively. The frequency of increased radiosensitivity based on this index (at 61% cut-off point), was 65.6% in the patients and about 33% in the control group.
Conclusion: The increased level of chromosome breakage following irradiation with gamma rays can be used as an early diagnostic biomarker of breast cancer or a possible indicator for breast cancer susceptibility.

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Article Type: Original Research | Subject: Sterility
Received: 2016/03/15 | Accepted: 2016/06/25 | Published: 2017/08/16

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