Volume 7, Issue 3 (2022)
SJMR 2022, 7(3): 165-170 |
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Nateghi M R, Amini Mahabadi J. Investigating the expression of mesenchymal stem cell markers in human bone marrow stem cells. SJMR 2022; 7 (3) : 5
URL: http://saremjrm.com/article-1-275-en.html
URL: http://saremjrm.com/article-1-275-en.html
1- Sarem gynecology, Obstetrics and Infertilty Research Center, Sarem Women's Hospital, Iran University of Medical Sciences (IUMS), Tehran, Iran & Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran.
2- Anatomical Sciences Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran & Gametogenesis Sciences Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran
2- Anatomical Sciences Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran & Gametogenesis Sciences Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran
Abstract: (1429 Views)
Introduction: Stem cells are undifferentiated cells that have the ability to convert and differentiate into different types of cells. Mesenchymal stem cells are multipotent cells that can differentiate into all types of cells. The purpose of this study was to cultivate and isolate mesenchymal stem cells from human bone marrow and to express some surface markers in mesenchymal stem cells.
Materials and Methods: In this research, human bone marrow sample (iliac crest) was used to produce bone marrow stromal cells. Bone marrow mesenchymal stem cells were isolated based on their adhesion properties. In order to prove that the cells are mesenchymal, in addition to their adhesion properties, CD13, CD49e, CD73, CD105 and CD90 markers were investigated after the fourth passage by flow cytometry.
Results: In the culture of cells derived from bone marrow up to the fourth passage, the mesenchymal cells were morphologically more similar to fibroblastic cells. Flow cytometry analysis of surface markers demonstrated that bone marrow mesenchymal stem cells from the fourth passage express CD13, CD49e and CD105 markers to a higher extent and CD73 and CD90 markers to a lesser extent.
Conclusion: Stem cells isolated from bone marrow have high levels of expression of surface markers specific to stem cells, especially of mesoderm origin, which proves these cells are mesenchymal.
Materials and Methods: In this research, human bone marrow sample (iliac crest) was used to produce bone marrow stromal cells. Bone marrow mesenchymal stem cells were isolated based on their adhesion properties. In order to prove that the cells are mesenchymal, in addition to their adhesion properties, CD13, CD49e, CD73, CD105 and CD90 markers were investigated after the fourth passage by flow cytometry.
Results: In the culture of cells derived from bone marrow up to the fourth passage, the mesenchymal cells were morphologically more similar to fibroblastic cells. Flow cytometry analysis of surface markers demonstrated that bone marrow mesenchymal stem cells from the fourth passage express CD13, CD49e and CD105 markers to a higher extent and CD73 and CD90 markers to a lesser extent.
Conclusion: Stem cells isolated from bone marrow have high levels of expression of surface markers specific to stem cells, especially of mesoderm origin, which proves these cells are mesenchymal.
Article number: 5
Article Type: Original Research |
Subject:
Reproduction
Received: 2022/11/6 | Accepted: 2022/12/6 | Published: 2023/06/14
Received: 2022/11/6 | Accepted: 2022/12/6 | Published: 2023/06/14
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