Volume 3, Issue 2 (2018)                   SJMR 2018, 3(2): 99-103 | Back to browse issues page

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Paknahad B, Saadat nia G, Mirza Ahamadi S, Salehian P. +14bp/-14bp Polymorphism of HLA-G Gene in Iranian Women with Recurrent Spontaneous Abortions. SJMR 2018; 3 (2) :99-103
URL: http://saremjrm.com/article-1-61-en.html
+14bp/-14bp Polymorphism of HLA-G Gene in Iranian Women with Recurrent Spontaneous Abortions
B. Paknahad1 , G. Saadat nia *2 , S. Mirza Ahamadi3 , P. Salehian4
1- Biotechnology Department, Iranian Research Organization for Scienceand Technology(IROST), Tehran, Iran
2- Biotechnology Department, Iranian Research Organization for Scienceand Technology (IROST), Tehran, Iran , gitasaadat@irost.ir
3- Basic Sciences Faculty, Zanjan Branch, Islamic Azad University, Zanjan, Iran
4- Sarem Fertility & Infertility Research Center (SAFIR), Sarem Women’s Hospital, Tehran, Iran
Abstract:   (6120 Views)

Aims: Spontaneous abortion is one of the most common complications of pregnancy. Human leukocyte antigen G (HLA-G) is thought to have a mediating role in mother and fetus interactions. The aim of this study was to investigate the association of the +14bp/-14bp polymorphism of HLA-G gene with recurrent spontaneous abortion (RSA) among Iranian women.
Materials and Methods: This case-control study was conducted on 50 patients with RSA and 50 normal women referred to Sarem women hospital (Tehran, Iran) during a six-month period from October 2012 to March 2013. After preparation of the blood samples and extraction of DNA, the exon 8 of HLA-G gene was amplified by polymerase chain reaction (PCR) and observed by polyacrylamide gel electrophoresis. Statistical analysis of data was done by Chi-square test using SPSS software.
Findings: There was a significant difference in the frequency of homozygous genotypes between patient and control groups (p=0.034). The homozygous genotypes of +14bp or -14bp were found more frequently in patients with RSA (60%) than the control group (34%). The odds ratio (OR) for homozygous genotypes relative to heterozygote genotype was found as 2.91.
Conclusion: The frequencies of heterozygous and homozygous genotypes of the polymorphism, +14bp/-14bp of the HLA-G gene, differ between Iranian women with a history of RSA and healthy women.

Keywords: +14bp/-14bp Polymorphism, HLA-G Gene, Abortion, Habitual, HLA-G Antigens
Full-Text [PDF 482 kb]   (2015 Downloads)    
Article Type: Original Research | Subject: Reproduction
Received: 2017/03/17 | Accepted: 2017/06/22 | Published: 2018/08/23
English Expanded Abstract [HTML 19 KB]  (149 Download)
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