Volume 2, Issue 4 (2017)
SJMR 2017, 2(4): 19-23 |
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Saremi A, Sanaye Naderi M, Pooladi A, Younesi B, Lashgari P, Zare A. Evaluations of WBC Cross-match Results after Lymphocyte Immunization in Women with Recurrent Spontaneous Abortion in Sarem Women’s Hospital. SJMR 2017; 2 (4) :19-23
URL: http://saremjrm.com/article-1-52-en.html
URL: http://saremjrm.com/article-1-52-en.html
1- “Sarem Fertility & Infertility Research Center (SAFIR)” and “Sarem Cell Research Center (SCRC)”, Sarem Women’s Hospital, Tehran, Iran
2- Sarem Fertility & Infertility Research Center (SAFIR), Sarem Women’s Hospital, Tehran, Iran
3- “Sarem Fertility & Infertility Research Center (SAFIR)” and “Sarem Cell Research Center (SCRC)”, Sarem Women’s Hospital, Tehran, Iran , azare@razi.tums.ac.ir
2- Sarem Fertility & Infertility Research Center (SAFIR), Sarem Women’s Hospital, Tehran, Iran
3- “Sarem Fertility & Infertility Research Center (SAFIR)” and “Sarem Cell Research Center (SCRC)”, Sarem Women’s Hospital, Tehran, Iran , azare@razi.tums.ac.ir
Abstract: (46820 Views)
Aims: Recurrent spontaneous abortion (RSA) is a complication in pregnancy that can results in fetus rejection by mother. Lymphocyte therapy is considered as a treatment method for such patients. Leukocyte cross-match test is a method to evaluate the outcomes of lymphocyte therapy. The objective of this study was to evaluate the leukocyte cross-match results after lymphocyte immunization in women with RSA.
Materials & Methods: This descriptive and cross-sectional study was conducted on 704 volunteer subjects with RSA history referred to Sarem women’s hospital (Tehran, Iran) from 2009 to 2013. The peripheral blood mononuclear cells (PBMCs) were isolated from their husband or a third donor and were injected into the patients, two or three times. Two weeks after the last immunization, the patients’ sera were tested for anti-paternal cytotoxic antibodies (APCAs) by leukocyte cross-match test. The leukocyte cross-match percentages were considered as positive if its values were more than 35% and vice versa.
Findings: A positive result of leukocyte cross-match test was found in 319 (45.31%) patients with RSA that received paternal PBMCs two times. Immunization was repeated for the patients with negative tests using paternal or third donor PBMCs and positive result were observed in 219 (31.11%) patients. Totally, positive results were found in 538 (76.42%) patients.
Conclusion: Lymphocyte therapy can enhances the production of APCAs in patients with RSA. It can increase the possibility of positive cross match test in these patients when it is repeated for three times.
Article Type: Original Research |
Subject:
Sterility
Received: 2016/06/11 | Accepted: 2016/06/11 | Published: 2017/06/12
Received: 2016/06/11 | Accepted: 2016/06/11 | Published: 2017/06/12
English Expanded Abstract [HTML 20 KB] (204 Download)
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