Volume 9, Issue 3 (2024)                   SJMR 2024, 9(3): 189-195 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Talebian N. The Effect of Freezing and Thawing on the Viability and Motility of Human Sperm: A Review Study. SJMR 2024; 9 (3) : 8
URL: http://saremjrm.com/article-1-342-en.html
Sarem Gynecology, Obstetrics and Infertility Research Center, Sarem Women’s Hospital, Iran University of Medical Science (IUMS), Tehran, Iran. & Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran.
Abstract:   (3951 Views)
The cryopreservation of human spermatozoa has been an option for patients undergoing chemo or radiotherapies since the late 1950s. Cryopreservation of spermatozoa is a widely used technique, sperm cryopreservation is increasingly effective even in cases of other disorders, such as autoimmune diseases, that affect reproductive function. Moreover, sperm cryopreservation is offered to patients with severe oligospermia or ejaculatory dysfunction for intracytoplasmic sperm injection (ICSI). Also, some non-malignant diseases, such as diabetes and autoimmune disorders, may lead to testicular damage, and cryopreservation is also advisable in these conditions. Presently, there are different techniques for the cryopreservation of spermatozoa. Although there have been many improvements, the ideal technique for achieving better post-thaw sperm quality remains a mystery. A major obstacle during cryopreservation is the formation of intracellular ice crystals. Cryodamage generated by cryopreservation causes structural and molecular alterations in spermatozoa. Injuries can happen because of oxidative stress, temperature stress, and osmotic stress, resulting in changes in the plasma membrane fluidity, motility, viability, and DNA integrity of the spermatozoa. There are several methods for freezing and thawing semen, which may cause damage to sperm function, viability, and finally, quality and fertility. In this article, the effects of freezing and thawing on the viability and motility of sperm are reviewed. The possibility of cryopreserving human spermatozoa has existed for over 80 years now, and empirical methods are still used today. A common goal of all techniques used is to achieve the highest post-thaw cell survival possible because the cells face many obstacles during freezing, and cryoinjuries can happen. In summary, sperm cryopreservation is an essential technique, it can be observed in different cellular functions and levels, but it has many negative effects on sperm parameters because it causes a major decline in DNA integrity, membrane viability, motility, viability, and increases in the production of reactive oxygen species (ROS). With all articles reviewed, there is no certain conclusion concerning prioritizing a specific method, but we can assume that there is a bright future ahead for optimizing cryopreservation methods to a point at which we can achieve better fertility outcomes and higher pregnancy rates. Researchers are discovering new means of improving post-thaw sperm parameters.
Article number: 8
Full-Text [PDF 1022 kb]   (1305 Downloads)    
Article Type: Systematical Review | Subject: Sterility
Received: 2024/11/2 | Accepted: 2024/12/10 | Published: 2025/02/3

References
1. Hezavehei, et.al. Sperm servation: A review on current molecular cryobiology and advanced approaches. Reprod. Biomed. Online, 2018:37(3); 327-39 [DOI:10.1016/j.rbmo.2018.05.012] [PMID]
2. Ram Dayal, et al. Effect of Semen Freezing and Thawing on Sperm Survival and Motility Rate: A Comparative Analysis 2021:511-517 [DOI:10.32628/IJSRST218483]
3. Surg Lt, et al. Study of human sperm motility post cryopreservation. Medical Services, Armed Forces.2014:349-353 [DOI:10.1016/j.mjafi.2014.09.006] [PMID] []
4. Majzoub A, Agarwal A. Antioxidants in sperm cryopreservation. Male Infertility. 2020:671-8 [DOI:10.1007/978-3-030-32300-4_54]
5. Albrizio M, et al. Localization and functional modification of L-type voltage-gated calcium channels in equine spermatozoa from fresh and frozen semen. Theriogenology. 2015: 83(3): 421-9 [DOI:10.1016/j.theriogenology.2014.10.005] [PMID]
6. Benson, J, et al. The cryobiology of spermatozoa. Theriogenology 2012: 78, 1682-1699. [DOI:10.1016/j.theriogenology.2012.06.007] [PMID]
7. Amidi F, Pazhohan A, et al. The role of antioxidants in sperm freezing: a review. Cell Tissue Bank. 2016: 17(4): 745-756. [DOI:10.1007/s10561-016-9566-5] [PMID]
8. Bateni Z, Azadi L, et al. Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function. Syst Biol Reprod Med. 2014: 60 (4): 245-50. [DOI:10.3109/19396368.2014.897773] [PMID]
9. Azadi L, Tavalaee M, et al. Effects of Tempol and Quercetin on Human Sperm Function after Cryopreservation. Cryo Letters. 2017: 38(1):
10. Sanja Ozimic, et al. Sperm Cryopreservation Today: Approaches, Efficiency, and Pitfalls Mol. Biol. 2023:45, 4716-4734 [DOI:10.3390/cimb45060300] [PMID] []
11. Di Santo, et al. Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART. Adv Urol, 2012: 854837. [DOI:10.1155/2012/854837] [PMID] []
12. Anger JT, et al. Cryopreservation of sperm: indications, methods and results. J. urol. 2003:.170(4); 1079-84 [DOI:10.1097/01.ju.0000084820.98430.b8] [PMID]
13. Justice T, Christensen G. Sperm cryopreservation methods. Spermatogenesis. 2013: 209-15. [DOI:10.1007/978-1-62703-038-0_18] [PMID]
14. Martins, et al. Sperm cryopreservation. In vitro fertilization: 2019: 625-42 [DOI:10.1007/978-3-319-43011-9_51]
15. Said TM, et al. Implication of apoptosis in sperm cryoinjury. Reprod Biomed Online. 2010: 21(4): 456-62 [DOI:10.1016/j.rbmo.2010.05.011] [PMID]
16. Ibrar Muhammad Khan, et al. Impact of Cryopreservation on Spermatozoa Freeze-Thawed Traits and Relevance OMICS to Assess Sperm Cryo-Tolerance in Farm Animals.2021:volum8 [DOI:10.3389/fvets.2021.609180] [PMID] []
17. Li,Y-x, et al. Vitrification and conventional freezing methods in sperm cryopreservation: A systematic review and metaanalysis. Eur.J.Obstet.Gynecol, 2019: 233;84-92 [DOI:10.1016/j.ejogrb.2018.11.028] [PMID]
18. Li, Z, et al. Update on techniques for cryopreservation of human spermatozoa. Asian J. Androl. 2022: 24, 563 [DOI:10.4103/aja20229] [PMID] []
19. Sharma R, et al. Effect of sperm storage and selection techniques on sperm parameters. Syst Biol Reprod Med. 2015: 61(1):1-1 [DOI:10.3109/19396368.2014.976720] [PMID]
20. Desrosiers P, et al. Membranous and structural damage that occur during cryopreservation of human sperm may be time-related events. Fertil. Steril. 2006:85, 1744-1752 [DOI:10.1016/j.fertnstert.2005.11.046] [PMID]
21. Sanja Ozimic, et al. Sperm Cryopreservation Today: Approaches, Efficiency, and Pitfalls. Issues Mol. Biol. 2023: 45, 4716-4734ه [DOI:10.3390/cimb45060300] [PMID] []
22. Li, Z, et al. Update on techniques for cryopreservation of human spermatozoa. Asian J. Androl. 2022: 24, 563 [DOI:10.4103/aja20229] [PMID] []
23. Paoli, D, et al. Sperm Cryopreservation: Effects on Chromatin Structure. Genet. Damage Hum. Spermatozoa 2014: 791, 137-150. [DOI:10.1007/978-1-4614-7783-9_9] [PMID]
24. Yeste M. Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs. Theriogenology. 2016; 85(1): 47-64 [DOI:10.1016/j.theriogenology.2015.09.047] [PMID]
25. Pegg DE. Principles of Cryopreservation. Cryopreserv. Free. Dry. Protoc. 2015, 1257, 3-19 [DOI:10.1007/978-1-4939-2193-5_1] [PMID]
26. Isachenko V, et al. Vitrification of Human ICSI/IVF Spermatozoa without Cryoprotectants: New Capillary Technology. J. Androl. 2012:33, 462-468, [DOI:10.2164/jandrol.111.013789] [PMID]
27. Anger JT, Gilbert BR, Goldstein M. Cryopreservation of Sperm: Indications, Methods and Results. J. Urol. 2003, 170, 1079-1084. [DOI:10.1097/01.ju.0000084820.98430.b8] [PMID]
28. Kidd SA, et al. Effects of male age on semen quality and fertility: a review of the literature. Fertility and Sterility2001; 75(2):237-48 [DOI:10.1016/S0015-0282(00)01679-4] [PMID]
29. Ozkavukcu S, et al. Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. Journal of assisted reproduction and genetics. 2008; 25:403-11 [DOI:10.1007/s10815-008-9232-3] [PMID] []
30. Barratt CLR, et al. special interest group for andrology basic semen analysis course: A continued focus on accuracy, quality, efficiency and clinical relevance. Hum. Reprod. 2011, 26, 3207-3212. [DOI:10.1093/humrep/der312] [PMID]
31. Björndahl, L. The usefulness and significance of assessing rapidly progressive spermatozoa. Asian J. Androl. 2010, 12, 33-35. [DOI:10.1038/aja.2008.50] [PMID] []
32. O'Connell M, et al. The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Hum. Reprod. 2002, 17, 704-709. [DOI:10.1093/humrep/17.3.704] [PMID]
33. Flores E, et al. Cryopreservation-induced alterations in boar spermatozoa mitochondrial function are related to changes in the expression and location of midpiece mitofusin-2 and actin network.theriogenology.2010.:74(3):354-63 [DOI:10.1016/j.theriogenology.2010.02.018] [PMID]
34. Agha-Rahimi A, et al. Cryoprotectant-free, vitrification of human spermatozoa in new artificial seminal fluid. Andrology. 2016; 4(6): 1037-1044 [DOI:10.1111/andr.12212] [PMID]
35. Tavalaee M, et al. Effect of varicocelectomy on sperm functional characteristics and DNA methylation. Andrologia. 2015; 47(8): 904-9 [DOI:10.1111/and.12345] [PMID]
36. Karakus, FN, et al. Effect of curcumin on sperm parameters after the cryopreservation. Eur. J. Obstet. Gynecol Reprod. Biol. 2021, 267, 161-166. [DOI:10.1016/j.ejogrb.2021.10.027] [PMID]
37. Naeini ZK, et al. Evaluation of ebselen supplementation on cryopreservation medium in human semen. Iran J Reprod Med 2014; 12:249-56
38. Rarani FZ, et al. Correlation between sperm motility and sperm chromatin/DNA damage before and after cryopreservation and the effect of folic acid and nicotinic acid on post-thaw sperm quality in normozoospermic men. Cell Tissue Bank 2019 [DOI:10.1007/s10561-019-09775-6] [PMID]
39. 28:1-12an abscess. Arch Gynecol Obstet. 1997;261(1):55-8

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | {Sarem Journal of Medical Research}

Designed & Developed by : Yektaweb