Volume 8, Issue 1 (2023)
SJMR 2023, 8(1): 25-35 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mazoochi T, Hassani Bafrani H. Evaluation of the Effect of the Diet of Female Wistar Rats Containing Sesame Seeds on Uterine Factors. SJMR 2023; 8 (1) : 4
URL: http://saremjrm.com/article-1-289-en.html
URL: http://saremjrm.com/article-1-289-en.html
1- Gametogenesis Sciences Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran.
Abstract: (1455 Views)
Introduction: Studies show that some antioxidants are effective in improving female infertility. Several antioxidant compounds exist in sesame seed. This study was designed and carried out to examine the effect sesame seed present in the diet, on reproductive system histology of female adult rat. The methods used were histomorphometric as well as measurement of hormone (LH, FSH) levels.
Materials and methods: This experimental study was carried out on 30 female adults Wistar rat, 180 gram weight, that were obtained from laboratory animal center at Kashan University of Medical Sciences. Rats were divided randomly into experimental and control groups. The control group received standard diet and experimental group received diet containing 70% standard diet and 30% sesame seed after weaning for 12 weeks. At the end of the study, body weight, left and right ovary weight, the number of atretic follicles, preantral and antral, the diameter of the mucosa of the uterus tube, Myometrium, endometrial glands and endometrium were evaluated. Also, serum FSH and LH concentrations were estimated. Data was analyzed by SPSS software and t-test. P< 0.05 was considered as significant level.
Results: Body weight rats, left ovary weight, the mean of antral follicles, FSH and T levels, the diameter mean of the mucosa of the uterus tube, myometrium, endometrial glands and endometrium were not significant (P<0.05).
Right ovary weight, the number of atretic and preantral follicles in in the experimental group showed a significant increase compared with the control group (P<0.05).
Conclusion: This is the first study which has evaluated the histology effect of sesame seed on reproductive system of female adult Wistar rat. It can be concluded that sesame seed can improve the female fertility potentials.
Materials and methods: This experimental study was carried out on 30 female adults Wistar rat, 180 gram weight, that were obtained from laboratory animal center at Kashan University of Medical Sciences. Rats were divided randomly into experimental and control groups. The control group received standard diet and experimental group received diet containing 70% standard diet and 30% sesame seed after weaning for 12 weeks. At the end of the study, body weight, left and right ovary weight, the number of atretic follicles, preantral and antral, the diameter of the mucosa of the uterus tube, Myometrium, endometrial glands and endometrium were evaluated. Also, serum FSH and LH concentrations were estimated. Data was analyzed by SPSS software and t-test. P< 0.05 was considered as significant level.
Results: Body weight rats, left ovary weight, the mean of antral follicles, FSH and T levels, the diameter mean of the mucosa of the uterus tube, myometrium, endometrial glands and endometrium were not significant (P<0.05).
Right ovary weight, the number of atretic and preantral follicles in in the experimental group showed a significant increase compared with the control group (P<0.05).
Conclusion: This is the first study which has evaluated the histology effect of sesame seed on reproductive system of female adult Wistar rat. It can be concluded that sesame seed can improve the female fertility potentials.
Article number: 4
Article Type: Historical Research |
Subject:
Sterility
Received: 2023/05/12 | Accepted: 2023/06/10 | Published: 2023/10/10
Received: 2023/05/12 | Accepted: 2023/06/10 | Published: 2023/10/10
References
1. Sainath, S.B., et al., Protective role of Centella asiatica on lead-induced oxidative stress and suppressed reproductive health in male rats. Environ Toxicol Pharmacol, 2011. 32(2): 146-154. [DOI:10.1016/j.etap.2011.04.005]
2. Sharpe, R.M. and N.E. Skakkebaek, Are oestrogens involved in falling sperm counts and disorders of the male reproductive tract? The Lancet, 1993. 341(8857): 1392-1396. [DOI:10.1016/0140-6736(93)90953-E]
3. Shittu, L., et al., Sesame leaves intake improve and increase epididymal spermatocytes reserve in adult male Sprague Dawley rat. Scientific Research and Essays, 2007. 2(8): 319-324.
4. Arumughan, C., A. Jayalekshmy, and K. Suja, Process for extraction of antioxidants from sesame seed/cake. 2003.
5. Konan, A.B., J.Y. Datté, and P.A. Yapo, Nitric oxide pathway-mediated relaxant effect of aqueous sesame leaves extract (Sesamum radiatum Schum. & Thonn.) in the guinea-pig isolated aorta smooth muscle. BMC complementary and alternative medicine, 2008. 8(1): 23. [DOI:10.1186/1472-6882-8-23]
6. Kim, J.Y., D.S. Choi, and M.Y. Jung, Antiphoto-oxidative activity of sesamol in methylene blue-and chlorophyll-sensitized photo-oxidation of oil. Journal of agricultural and food chemistry, 2003. 51(11): 3460-3465. [DOI:10.1021/jf026056p]
7. Jeng, K. and R. Hou, Sesamin and Sesamolin: Natures therapeutic lignans. Current Enzyme Inhibition, 2005. 1(1): 11-20. [DOI:10.2174/1573408052952748]
8. Einer-Jensen, N. and R. Hunter, Physiological and pharmacological aspects of local transfer of substances in the ovarian adnexa in women. Human Reproduction Update, 2000. 6(2): 132-138. [DOI:10.1093/humupd/6.2.132]
9. Marcondes, F., F. Bianchi, and A. Tanno, Determination of the estrous cycle phases of rats: some helpful considerations. Brazilian Journal of Biology, 2002. 62(4A): 609-614. [DOI:10.1590/S1519-69842002000400008]
10. Talsness, C.E., et al., In utero and lactational exposures to low doses of polybrominated diphenyl ether-47 alter the reproductive system and thyroid gland of female rat offspring. Environmental Health Perspectives, 2008. 116(3): 308. [DOI:10.1289/ehp.10536]
11. Tarıin, J., et al., Oral administration of pharmacological doses of vitamins C and E reduces reproductive fitness and impairs the ovarian and uterine functions of female mice. Theriogenology, 2002. 57(5): 1539-1550. [DOI:10.1016/S0093-691X(02)00636-2]
12. Myers, M., et al., Methods for quantifying follicular numbers within the mouse ovary. Reproduction, 2004. 127(5): 569-580. [DOI:10.1530/rep.1.00095]
13. HIRSHFIELD, A.N. and A.R. MIDGLEY, Morphometric analysis of follicular development in the rat. Biology of reproduction, 1978. 19(3): 597-605. [DOI:10.1095/biolreprod19.3.597]
14. Odugbemi, T., A textbook of medicinal plants from nigeria. 2008: Tolu Odugbemi.
15. Jayasinghe, L., et al., Antifungal constituents of the stem bark of Bridelia retusa. Phytochemistry, 2003. 62(4): 637-641. [DOI:10.1016/S0031-9422(02)00623-4]
16. Mehranjani, M.S., et al., Effects of vitamin E on ovarian tissue of rats following treatment with p-nonylphenol: A stereological study. Iranian Journal of Reproductive Medicine, 2010. 8(1): 1-9.
17. مازوچی, et al., اثر اسیدآسکوربیک بر بیان ژنهای Bax و Bcl-2 و تکوین فولیکولهای پرهآنترال جدا شده از تخمدانهای انجمادی و غیرانجمادی در محیط کشت. دانشگاه علوم پزشکی بوشهر، طب جنوب, 2010. 13(3): 151-162.
18. Parvin Jahromi, S., et al., Histopathological and stereological studies of soybean hydroalcoholic extract effects on the rat ovary. ISMJ, 2012. 15(3): 161-170.
19. Güney, M., et al., Ovarian toxicity in rats caused by methidathion and ameliorating effect of vitamins E and C. Human & experimental toxicology, 2007. 26(6): 491-498. [DOI:10.1177/0960327106077505]
20. Braw, R.H. and A. Tsafriri, Effect of PMSG on follicular atresia in the immature rat ovary. Journal of reproduction and fertility, 1980. 59(2): 267-272. [DOI:10.1530/jrf.0.0590267]
21. Tilly, J.L., et al., Involvement of apoptosis in ovarian follicular atresia and postovulatory regression. Endocrinology, 1991. 129(5): 2799-2801. [DOI:10.1210/endo-129-5-2799]
22. Gu, J.-Y., et al., Effect of dietary fats and sesamin on the lipid metabolism and immune function of Sprague-Dawley rats. Bioscience, biotechnology, and biochemistry, 1998. 62(10): 1917-1924. [DOI:10.1271/bbb.62.1917]
23. Vicky, L.-C., et al., Sesamin Modulates Tyrosine Hydroxylase, Superoxide Dismutase, Catalase, Inducible No Synthase and Interleukin-6 Expression in Dopaminergic Cells Under Mpp+-Induced Oxidative Stress. Oxidative Medicine and Cellular Longevity, 1900. 1(1): 54-62. [DOI:10.4161/oxim.1.1.6958]
24. Pianjing, P., et al., Estrogenic activities of sesame lignans and their metabolites on human breast cancer cells. Journal of agricultural and food chemistry, 2010. 59(1): 212-221. [DOI:10.1021/jf102006w]
25. Parborell, F., et al., Effects of a gonadotropin-releasing hormone agonist on rat ovarian follicle apoptosis: regulation by epidermal growth factor and the expression of Bcl-2-related genes. Biology of reproduction, 2002. 67(2): 481-486. [DOI:10.1095/biolreprod67.2.481]
26. Ashamu, E., et al., Efficacy of vitamin C and ethanolic extract of Sesamum indicum in promoting fertility in male Wistar rats. Journal of human reproductive sciences, 2010. 3(1): 11. [DOI:10.4103/0974-1208.63115]
27. Morovvati, H., H. Najafzadeh, and K. Menatian, Effect of quercetin on uterus tissue histological changes in ovariectomized rats. 2013.
28. Tsuji, M., et al., Dietary quercetin inhibits bone loss without effect on the uterus in ovariectomized mice. Journal of bone and mineral metabolism, 2009. 27: 673-681. [DOI:10.1007/s00774-009-0088-0]
29. Wang, C.C., et al., Prodrug of green tea epigallocatechin-3-gallate (Pro-EGCG) as a potent anti-angiogenesis agent for endometriosis in mice. Angiogenesis, 2013. 16(1): 59-69. [DOI:10.1007/s10456-012-9299-4]
30. Mahabadi, J.A., H.H. Bafrani, and H. Nikzad, Effect of Sesame-Supplemented Diet on Prostate and Seminal Vesicle Histology of Adult Rat. International Journal of M
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
