Volume 6, Issue 4 (2021)                   SJMR 2021, 6(4): 237-247 | Back to browse issues page


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Pazhoomand R, Keihani E, Behjati F. Expression of ERBB1 gene in Iranian women with sporadic breast cancer and its relationship with clinicopathological features of the disease. SJMR 2021; 6 (4) : 6
URL: http://saremjrm.com/article-1-244-en.html
1- Genetic Laboratory of Shiraz Fertility Center, Shiraz, Iran.Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran.
2- Associate Professor, Clinical Research Development Center of Rofeideh Rehabilitation Hospital- University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
3- Sarem Fertility and Infertility Research Center (SAFIR), Sarem Women’s Hospital, Iran University of Medical Science (IUMS), Tehran, Iran & Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran & Genetics Research Centre, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran. , fbehjati@gmail.com
Abstract:   (1227 Views)
Introduction: ERBB1 gene is highly expressed in various human tumors, including breast cancer, and is considered as the initiator of various important stages during malignancy. The aim of this study was to investigate of mutation and expression of the ERBB1 gene in Iranian women with primary breast cancer and its relationship with the pathobiology characteristics of the disease.
Material and methods: Women with primary breast cancer were admitted to Mehrad Hospital in Tehran and underwent surgery. Part of the macroscopically visible tissue sample was collected from 121 patients with this cancer. 81 patients were appropriate for entry into the study. DNA and RNA were extracted. ERBB1 copy number was investigated using the MLPA technique. ERBB1 RNA was extracted from the tissue samples and cDNA synthesis was performed with a kit. Samples were analyzed using Real-time PCR for the determination of ERBB1 gene expression level.
Results: The age range of the patients was between 41 and 70 years, and the patients were divided into two categories, below and above 50 years. In 7 patients with ERBB1 copy increase, none showed increased expression for this gene. Also, five patients who had an increase in the expression of this gene did not show an increase in the number of copies of this gene. No statistically significant relationship was observed between increased expression and copy number of this gene in the patients. But; there was significant association between ERBB1 gene expression and III-I stage of the disease (P<0.001); however, there was no significant association between clinical and histopathological parameters with ERBB1 gene mutation and expression.
Conclusion: This study shows that an increase in the expression of the ERBB1 gene does not necessarily reflect an increase in copy number of the gene. Other mechanisms such as epigenetics might be involved. There was significant association between ERBB1 gene expression and III-I stage of disease. However, these findings should be investigated in a larger sample size.
Article number: 6
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Article Type: Original Research | Subject: Women Diseases
Received: 2022/10/23 | Accepted: 2022/12/13 | Published: 2022/12/13

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