Volume 3, Issue 2 (2018)                   SJRM 2018, 3(2): 93-97 | Back to browse issues page


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Roumandeh N, Saremi A, Sanaye Naderi M, Younesi B, Arasteh J, Zare A. Comparison of Cytokines Levels Released from Th17 and Regulatory T Cells in Patients with Recurrent Spontaneous Abortions and Healthy Women. SJRM. 2018; 3 (2) :93-97
URL: http://saremjrm.com/article-1-60-en.html
1- *“Sarem Fertility & Infertility Research Center (SAFIR), Sarem Cell Research Center (SCRC)” Sarem Women’s Hospital” and “Asthma and Allergy Immunology Research Center, Tehran University of Medical Sciences”, Tehran, Iran
2- “Sarem Fertility & Infertility Research Center (SAFIR)” and “Sarem Cell Research Center (SCRC)”, Sarem Women’s Hospital, Tehran, Iran
3- Sarem Fertility & Infertility Research Center (SAFIR), Sarem Women’s Hospital, Tehran, Iran
4- Sarem Women’s Hospital, Tehran, Iran
5- Biology Department, Basic Sciences Faculty, Central Tehran Branch, Islamic Azad University, Tehran, Iran
6- *“Sarem Fertility & Infertility Research Center (SAFIR), Sarem Cell Research Center (SCRC)” Sarem Women’s Hospital” and “Asthma and Allergy Immunology Research Center, Tehran University of Medical Sciences”, Tehran, Iran , zare-a@alumnus.tums.ac.ir
Abstract:   (2711 Views)

Aims: Unexplained recurrent spontaneous abortion (URSA) might be caused by an immunological rejection of fetus due to the immune dysregulation during pregnancy. It is supposed that the balance between Th17 and regulatory T cells (Treg) is one of the immune system regulatory mechanisms for pregnancy. The objective of the present study was to evaluate the Treg and Th17 cytokines in the women with URSA compared to healthy women.
Materials and Methods: This case-control study was carried out on 30 women with a history of three or more URSA (case group) and 28 normal healthy women with at least one successful delivery (control group) in Sarem women hospital (Tehran, Iran). The levels of IL-17, IL-21, IL-10 and TGF-β were measured in the serum samples by ELISA method and compared between two groups. The data were analyzed by Mann-Whitney U test and Spearmanchr('39')s rank correlation coefficient using SPSS 22 software.
Findings: The level of IL-17 in the case group was significantly higher than the control group (p<0.001). Also, the level of TGF-β in the control group was significantly higher than URSA patients (p=0.001). The serum level of IL-17 showed a positive correlation with TGF-β in URSA group (r=0.554; p=0.002). There were no significant differences in IL-21 and IL-10 levels between two groups (p>0.05).
Conclusion: The level of IL-17 in patients with URSA is higher than normal healthy women, however, the concentration of TGF-β in women with URSA is lower than healthy women; these findings show that Th17 immunity and regulatory T cell-mediated immune regulation are involved in the pathogenesis of URSA.

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Article Type: Original Research | Subject: Reproduction
Received: 2017/02/8 | Accepted: 2017/06/28 | Published: 2018/08/23

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