Volume 1, Issue 1 (2017)                   SJRM 2017, 1(1): 35-38 | Back to browse issues page

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Hassani M, Bagher K. 27 Weeks Fetus with Cardiomegaly, Hydrocephaly and Pathological Diagnosis of Cytomegalovirus. SJRM. 2017; 1 (1) :35-38
URL: http://saremjrm.com/article-1-31-en.html
1- Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran , mha1353@gmail.com
2- General Sarem Lab, Sarem Women’s Hospital, Tehran, Iran
Abstract:   (3964 Views)

Introduction: Cytomegalovirus (CMV) is a member of the β-Herpes family, which produces cytomegalic inclusions in infected cells. This virus produces a syndrome similar to infectious mononucleosis. Presence of specific antibodies against Cytomegalovirus in persons ‘ serum shows that, people contact with CMV during their lifetime. The first encounter with this virus during pregnancy can cause abnormalities such as cardiomegaly, hydrocephalus, and even fetal death.
Patient Information: This case report concerns a 27 weeks fetus from a 27 year old mother at Sarem Hospital. According to the pathologic findings of the samples stained with H&E and PAS methods, molecular tests were performed to detect Cytomegalovirus, and after DNA extraction from paraffin blocks obtained from different tissues of the fetus using Nested-PCR with two pairs of primers (CMTR1, CMTR2, CMTR3, and CMTR4), the presence of this virus was investigated in two stages.
Conclusion: Regarding the healthy karyotype and the absence of genetic problems and molecular investigations, the main cause of the changes in the fetus, including cardiomegaly, hydrocephalus, and the cause of fetal death in the 27th week, is the presence of Cytomegalovirus.

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Article Type: Case Report | Subject: Sterility
Received: 2015/09/1 | Accepted: 2016/02/15 | Published: 2016/12/25

1. Hunt R. Microbiology and immunology online [Internet]. University of south Carolina school of Medicine:1801. Oncogenic viruses-DNA tumor viruses; [updated 2016 jun5; cited 2016 jul14]. Available from: http://www.microbiologybook.org/book/virol-sta.htm [Link]
2. Jawetz MA. Medical Microbiology. Norouzi J, Translator. Tehran: Hayyan Publishing Cultural Institute; 2000. [Persian] [Link] [PMID]
3. Stagno S, Pass RF, Dworsky ME, Henderson RE, Moore EG, Walton PD, et al. Congenital cytomegalovirus infection: the relative importance of primery and recurrent maternal infections. N Engl J Med. 1982;306(16):945-9. [Link] [DOI:10.1056/NEJM198204223061601] [PMID]
4. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988;239(4839):487-91. https://doi.org/10.1126/science.2448875 [Link] [DOI:10.1126/science.239.4839.487]
5. Schochetman G, Ou CY, Jones WK. Polymerase chain reaction. J Infect Dis. 1988;158(6):1154-7. [Link] [DOI:10.1093/infdis/158.6.1154]
6. Khan G, Kangro HO, Coates PJ, Heath RB. Inhibitory effects of urine on the polymerase chain reaction for cytomegalovirus DNA. J Clin Pathol. 1991;44(5):360-5. [Link] [DOI:10.1136/jcp.44.5.360]
7. Razzaque A, Jahan N, MC Weeney D, etal. Localization of DNA sequence analysis of the transforming domain (mtrII) of human cytomegalovirus. Proc Natl Acad Sci USA. 1988;85(15):5709-13. [Link] [DOI:10.1073/pnas.85.15.5709]
8. Cytomegalovirus (CMV) and Congenital CMV Infection [Internet]. Atlanta: Centers for Disease control and prevent; 1946. [updated 2017 dec 5; cited 2008 jun 1]. Avilable from: https://www.cdc.gov/cmv/overview.html. [Link]
9. Pickring LK, Baker CF, Long SS, McMillan JA, editors. Red Book: 2006 Report of the Committee on Infectious Diseases. Elk Grove Village: American Academy of Pediatrics. 2006. [Link]
10. Lo SK, Ip KW, Chan PK, Nicholls JM, Heath RB, Shiu SY. Congenital infection by human cytomegalovirus with a 65bp deletion in the morphological transforming region II. Arch Virol. 1993;129(1-4):295-9. [Link] [DOI:10.1007/BF01316904] [PMID]
11. Wright DK, Manos MM. Sample preparation from paraffin-embedded tissues. In: Inns MA, Gelfand DH, Srinsky JJ, White TJ. PCR protocols: A guide to method and applications. San Diego: Academic Press; 1990. p. 153-8. [Link] [DOI:10.1016/B978-0-12-372180-8.50023-8]

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