Volume 4, Issue 1 (2019)                   SJMR 2019, 4(1): 59-64 | Back to browse issues page

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Zare A, Saremi A, Salehian P, Roomandeh N, Naderi M, Lashgari P, et al . Effect of Lymphocyte Immunotherapy on Pro-Inflammatory Th17 Related Cytokine Levels in Women with Recurrent Spontaneous Abortion. SJMR. 2019; 4 (1) :59-64
URL: http://saremjrm.com/article-1-127-en.html
1- Sarem Cell Research Center, Sarem Women’s Hospital, Tehran, Iran , ahadzr@gmail.com
2- Sarem Cell Research Center, Sarem Women’s Hospital, Tehran, Iran
3- Cancer Research Center and Department of Immunology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
4- Department of Biology, Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Abstract:   (2152 Views)
Aims: Alterations of Th17 cells during pregnancy have a crucial role in maintenance of fetus and the high level of these cells has been observed in women with recurrent spontaneous abortion. Immunotherapy with paternal lymphocytes is one of the effective therapeutic methods for this complication. The aim of this study was to evaluate the alteration in Th17 cytokine levels in patients with recurrent abortion after lymphocyte immunotherapy.
Materials & Methods: In this semi-experimental study, 30 patients referred to Sarem hospital with a history of at least three recurrent abortions in 2013 were evaluated. The levels of IL-17and IL-21 in patient serum and supernatant of cultured peripheral blood mononuclear cells were assayed before and after this treatment by using ELISA. Statistical analysis was performed with Wilcoxon test by SPSS 22.
Findings: The levels of IL-17and IL-21 in patient serum after immunotherapy were significantly decreased (p<0.05). Supernatant of cultured peripheral blood mononuclear cells after immunotherapy showed a low level of IL-21 (p<0.04), while this change was not significant for interleukin 17 (p=0.13).
Conclusion: Lymphocyte immunotherapy reduces the level of pro-inflammatory cytokines of Th17 cells in women with recurrent spontaneous abortion.
Full-Text [PDF 444 kb]   (444 Downloads)    
Article Type: Brief Communication | Subject: Reproduction
Received: 2018/06/7 | Accepted: 2019/03/16 | Published: 2019/06/15

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