Volume 7, Issue 3 (2022)                   SJMR 2022, 7(3): 143-150 | Back to browse issues page


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Hassani Bafrani M, Amini Mahabadi A, Hassani Bafrani H. Investigating the Effect of Diet Containing Sesame Seeds on Epididymis and Seminal Vesicles of Adult Wistar Rats. SJMR 2022; 7 (3) : 2
URL: http://saremjrm.com/article-1-270-en.html
1- Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
2- Responsible for administrative affairs, Payame Noor University, Ardestan, Isfahan, Iran.
3-
Abstract:   (1386 Views)
Introduction: Infertility is one of the world's medical problems; about 10-15% of couples have experienced the problem of infertility. In recent years, many efforts have been made to identify a desirable and ideal medicinal plant with a strong anti-metabolic effect and the effect of this plant on male fertility. Sesame is an essential oil seed obtained from the Sesamum indicum plant. This study aimed to investigate the effect of the sesame seed diet on the epididymis and seminal vesicle structure of adult male rats.
Material and methods: The 30 adult Wistar rats weighing 200 gr were obtained from the animal house of Kashan University of Medical Sciences. Mice were randomly divided into two experimental groups (n=15) and control (n=15). The control group received a standard diet and the experimental group received a diet containing 70% of the standard diet and 30% of sesame seeds after weaning for 12 weeks. The right epididymis was removed and crushed into several pieces in a sample bottle containing normal saline for several minutes to allow the sperm to swim out. Sperm parameters, sperm count, and motility were also determined. The left epididymis was divided into three parts and fixed in Bouin's solution for histological evaluation. Also, seminal vesicles were examined after being fixed. The data were analyzed using SPSS software and the t-test. P<0.05 was considered significant.
Results: The average number of cells and sperm motility in the left epididymis in the experimental group was very significant compared to the control (P<0.001). The average diameter of the epididymis of the tubes, lumen and epithelium was not significantly different in the three parts (P>0.05). A significant difference was observed between the treatment and control groups in fibromuscular diameter and seminal vesicle epithelium (P<0.05). The volume density of the seminal vesicle epithelium in the treatment group compared to the control group increased significantly, but the volume density of the fibromuscular gland and the lumen of the seminal vesicle decreased significantly in the treatment group compared to the control group.
Conclusion: Sesame consumption improved sperm motility and number. However, it did not affect epididymal tissue. Therefore, to further investigate this seed on the reproductive system, more studies should be conducted in this field.
Article number: 2
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Article Type: Original Research | Subject: Sterility
Received: 2023/04/10 | Accepted: 2023/04/10 | Published: 2023/04/10

References
1. 1. Sainath, S., et al., Protective role of Centella asiatica on lead-induced oxidative stress and suppressed reproductive health in male rats. environmental toxicology and pharmacology, 2011. 32(2): p. 146-154. [DOI:10.1016/j.etap.2011.04.005]
2. Esmaeilzadeh, S., M. Farsi, and T. Nazari, The cause of infertility frequency in the patients referring to Babol township fatemeh zahra infertility center from May 1996 to May 1998. Journal of Mazandaran University of Medical Sciences, 2002. 12(35): p. 29-34.
3. Shittu, L., et al., Hypoglycaemia and improved testicular parameters in Sesamum radiatum treated normo-glycaemic adult male Sprague Dawley rats. African Journal of Biotechnology, 2009. 8(12).
4. Brar, G.S. and K. Ahuja, Sesame: it's culture, genetics, breeding and biochemistry. 1979: p. 245-313.
5. Agboola, S., An agricultural atlas of Nigeria. An agricultural atlas of Nigeria, 1979: p. 262.
6. FAO, A.C.A., Food and Agriculture Organization. Organización de Naciones Unidas: http://www. fao. org/ag/locusts/en/info/info/index. html, 2005.
7. Shittu, L., et al., Differential antimicrobial activity of the various crude leaves extracts of Sesame radiatum against some common pathogenic micro-organisms. Sci. Res. Essay, 2006. 1(3): p. 108-111. [DOI:10.2139/ssrn.3017601]
8. Tarıin, J., et al., Oral administration of pharmacological doses of vitamins C and E reduces reproductive fitness and impairs the ovarian and uterine functions of female mice. Theriogenology, 2002. 57(5): p. 1539-1550. [DOI:10.1016/S0093-691X(02)00636-2]
9. Einer-Jensen, N. and R. Hunter, Physiological and pharmacological aspects of local transfer of substances in the ovarian adnexa in women. Human Reproduction Update, 2000. 6(2): p. 132-138. [DOI:10.1093/humupd/6.2.132]
10. Krinke, G., The handbook of experimental animals. The laboratory rat. San Diego: Academic, 2000: p. chapter 9,145-152.
11. Hadley, M., Endocrinology 4th ,New Jersey Prentice Hall. 1998.
12. Świder-Al-Amawi, M., et al., The immunoexpression of FSH-R in the ductuli efferentes and the epididymis of men and rat: effect of FSH on the morphology and steroidogenic activity of rat epididymal epithelial cells in vitro. Journal of Biomedicine and Biotechnology, 2010. 2010. [DOI:10.1155/2010/506762]
13. Torres-Arce, E., et al., Dietary antioxidants in the treatment of male infertility: Counteracting oxidative stress. Biology, 2021. 10(3): p. 241. [DOI:10.3390/biology10030241]
14. Mitra, J. and M. Chowdhury, Association of glycerylphosphorylcholine with human sperm and effect of capacitation on their metabolism. Reproduction, fertility and development, 1994. 6(6): p. 679-685. [DOI:10.1071/RD9940679]
15. Hemalatha, S. and M. Raghunath, Dietary sesame (Sesamum indicum cultivar Linn) oil inhibits iron-induced oxidative stress in rats. British journal of nutrition, 2004. 92(04): p. 581-587. [DOI:10.1079/BJN20041239]
16. Jeng, K. and R. Hou, Sesamin and Sesamolin: Natures therapeutic lignans. Current Enzyme Inhibition, 2005. 1(1): p. 11-20. [DOI:10.2174/1573408052952748]
17. Yang, H.S., et al., Effects of α-tocopherol on cadmium-induced toxicity in rat testis and spermatogenesis. Journal of Korean medical science, 2006. 21(3): p. 445-451. [DOI:10.3346/jkms.2006.21.3.445]
18. 18 , Ashamu, E., et al., Efficacy of vitamin C and ethanolic extract of Sesamum indicum in promoting fertility in male Wistar rats. Journal of human reproductive sciences, 2010. 3(1): p. 11. [DOI:10.4103/0974-1208.63115]
19. 19, Mukerjee, B. and T. Rajan, Morphometric study of seminal vesicles of rat in normal health and stress conditions. J. Anat. Soc. India, 2006. 55(1): p. 37-42.
20. 20 ,. Wong, W.Y., et al., The impact of calcium, magnesium, zinc, and copper in blood and seminal plasma on semen parameters in men. Reproductive toxicology, 2001. 15(2): p. 131-136. [DOI:10.1016/S0890-6238(01)00113-7]
21. 21 ,. Nordberg, G., Effects of long-term cadmium exposure on the seminal vesicles of mice. Reproduction, 1975. 45(1): p. 165-167. [DOI:10.1530/jrf.0.0450165]

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