Volume 1, Issue 1 (2017)                   SJMR 2017, 1(1): 9-13 | Back to browse issues page

‎ 10.29252/sjrm.1.1.9

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Osteoblastic Differentiation of Amniotic Pluripotent Stem Cell
M. Mahmoudinia Meymand1 , M. Noruzinia * 2
1- Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran
2- Medical Genetics Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , noruzinia@modares.ac.ir
Abstract:   (6590 Views)

Aims: Amniotic fluid stem cells have lower ethical limitations than embryonic stem cells for the use in research and treatment. These cells show great self-renewal potential and can differentiate into the specialized cells of all three germ layers. The amniotic fluid stem cells display minimal risks of teratomas and very low immunogenicity. For these reasons, amniotic fluid appears as a promising alternative source for stem cell therapy. The objective of this study was to isolate the stem cells from amniotic fluid and differentiate them into the osteoblastic cells.
Materials and Methods: An amniotic fluid sample (about 10ml) was collected from a healthy donor in Sarem women’s hospital (Tehran, Iran). After centrifugation, the cells were cultured in a DMEM medium supplemented with 20% FBS. The cell clones were observed after two weeks and were passaged to an osteoblastic differentiation medium. Alizarin red staining and RT-PCR for alkaline phosphatase and osteocalcin markers were used for confirmation of cellular differentiation.
Findings: Stem cells were isolated from amniotic fluid. Phenotypically, these cells showed spindle-shaped morphology with a large nucleus. Following the induction of differentiation, they showed the expression of osteoblastic cells markers indicating their differentiation. The expression of those markers was confirmed by immunocytochemistry and RT-PCR.
Conclusion: Amniotic stem cells have the ability to differentiate into the osteoblastic cells using an osteoblastic differentiation medium.

Keywords: Fetal Stem Cells, Amniotic Fluid, Osteoblasts, Cell Differentiation
Full-Text [PDF 616 kb]   (1909 Downloads)    
Article Type: Analytical Review | Subject: Sterility
Received: 2015/09/27 | Accepted: 2016/01/12 | Published: 2017/02/14
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